In vitro Model of Hypoxia: Basic Fibroblast Growth Factor Can Rescue Cultured CNS Neurons from Oxygen-Deprived Cell Death-Reference-Cited by-同舟云学术

In vitro Model of Hypoxia: Basic Fibroblast Growth Factor Can Rescue Cultured CNS Neurons from Oxygen-Deprived Cell Death

Published:1993-11 Issue:6 Volume:13 Page:1029-1032
ISSN:0271-678X
Container-title:Journal of Cerebral Blood Flow & Metabolism
language:en
Short-container-title:J Cereb Blood Flow Metab

Author:

Akaneya Yukio¹²,Enokido Yasushi¹,Takahashi Mitsuo²,Hatanaka Hiroshi¹

Affiliation:

1. Division of Protein Biosynthesis, Institute for Protein Research, Osaka University

2. Department of Neurology, Kinki University School of Medicine, Osaka, Japan

Abstract

We established an in vitro hypoxia model and investigated the protective effect of basic fibroblast growth factor (bFGF) against neuronal cell death caused by hypoxia. Hippocampal neurons obtained from rats on embryonic day (E) 17 and 20 and on postnatal day (P) 4 were cultured for 6–24 h in an oxygen-deprived state. This in vitro hypoxia study showed that the cultured neurons were sensitive to the oxygen deprivation. The cultured P4 rat hippocampal neurons seemed to be weaker in the hypoxia condition than those of E17 and E20 rats, suggesting that the cultured postnatal cells might be sensitive to hypoxia. bFGF, but not nerve growth factor, prevented the neuronal cell death caused by hypoxia in a dose-dependent manner.

Publisher

SAGE Publications

Subject

Cardiology and Cardiovascular Medicine,Clinical Neurology,Neurology

Link

http://journals.sagepub.com/doi/pdf/10.1038/jcbfm.1993.130

Reference13 articles.

1. Glutamate neurotoxicity in cortical cell culture

2. Calcium Accumulation and Neuronal Damage in the Rat Hippocampus following Cerebral Ischemia

3. Regional Accumulation of Calcium in Postischemic Rat Brain

4. Basic fibroblast growth factor rescues CNS neurons from cell death caused by high oxygen atmosphere in culture

5. Differential effects of nerve-growth factor and glioma-conditioned medium on neurons cultured from various regions of fetal rat central nervous system

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