Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays-Reference-Cited by-同舟云学术

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Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays

Published:2006-09-21 Issue:10 Volume:3 Page:833-838
ISSN:1548-7091
Container-title:Nature Methods
language:en
Short-container-title:Nat Methods

Author:

Kulkarni Meghana M,Booker Matthew,Silver Serena J,Friedman Adam,Hong Pengyu,Perrimon Norbert,Mathey-Prevot Bernard

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,Biochemistry,Biotechnology

Link

http://www.nature.com/articles/nmeth935.pdf

Reference23 articles.

1. Hannon, G.J. RNA interference. Nature 418, 244–251 (2002).

2. Jackson, A.L. et al. Expression profiling reveals off-target gene regulation by RNAi. Nat. Biotechnol. 21, 635–637 (2003).

3. Scacheri, P.C. et al. Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells. Proc. Natl. Acad. Sci. USA 101, 1892–1897 (2004).

4. Birmingham, A. et al. 3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets. Nat. Methods 3, 199–204 (2006).

5. Doench, J.G. & Sharp, P.A. Specificity of microRNA target selection in translational repression. Genes Dev. 18, 504–511 (2004).

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