A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/srep37895.pdf
Reference50 articles.
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2. Gu, P. F. et al. A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies. Sci Rep-Uk 5, doi: ARTN968410.1038/srep09684 (2015).
3. Smanski, M. J. et al. Synthetic biology to access and expand nature’s chemical diversity. Nat Rev Microbiol 14, 135–149, doi: 10.1038/nrmicro.2015.24 (2016).
4. Esvelt, K. M. & Wang, H. H. Genome-scale engineering for systems and synthetic biology. Mol Syst Biol 9, 641, doi: 10.1038/msb.2012.66 (2013).
5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N. & Court, D. L. Recombineering: genetic engineering in bacteria using homologous recombination. Curr Protoc Mol Biol 106, 1 1611–1116 39, doi: 10.1002/0471142727.mb0116s106 (2014).
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