Abstract
AbstractBiofuel production by Clostridium acetobutylicum is compromised by strain degeneration due to loss of its pSOL1 megaplasmid. Here we used engineering biology to stably integrate pSOL1 into the chromosome together with a synthetic isopropanol pathway. In a membrane bioreactor continuously fed with glucose mineral medium, the final strain produced advanced biofuels, n-butanol and isopropanol, at high yield (0.31 g g−1), titre (15.4 g l−1) and productivity (15.5 g l−1 h−1) without degeneration.
Funder
Innovate UK
RCUK | Biotechnology and Biological Sciences Research Council
Publisher
Springer Science and Business Media LLC
Reference37 articles.
1. Jones, D. T. & Woods, D. R. Acetone–butanol fermentation revisited. Microbiol. Rev. 50, 484–524 (1986).
2. Weizmann, C. Improvements in the bacterialfFermentation of carbohydrates and in bacterial cultures for the same, GB patent 191504845A (1915).
3. Weizmann, C. Production of acetone and alcohol by bacteriological process, US patent 1315585A (1919).
4. Ndaba, B., Chiyanzu, I. & Marx, S. n-Butanol derived from biochemical and chemical routes: a review. Biotechnol. Rep. 8, 1–9 (2015).
5. Tracy, B. P., Jones, S. W., Fast, A. G., Indurthi, D. C. & Papoutsakis, E. T. Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications. Curr. Opin. Biotech. 23, 364–381 (2012).
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献