Abstract
AbstractMicrobial predators such as choanoflagellates are key players in ocean food webs. Choanoflagellates, which are the closest unicellular relatives of animals, consume bacteria and also exhibit marked biological transitions triggered by bacterial compounds, yet their native microbiomes remain uncharacterized. Here we report the discovery of a ubiquitous, uncultured bacterial lineage we name Candidatus Comchoanobacterales ord. nov., related to the human pathogen Coxiella and physically associated with the uncultured marine choanoflagellate Bicosta minor. We analyse complete ‘Comchoano’ genomes acquired after sorting single Bicosta cells, finding signatures of obligate host-dependence, including reduction of pathways encoding glycolysis, membrane components, amino acids and B-vitamins. Comchoano encode the necessary apparatus to import energy and other compounds from the host, proteins for host-cell associations and a type IV secretion system closest to Coxiella’s that is expressed in Pacific Ocean metatranscriptomes. Interactions between choanoflagellates and their microbiota could reshape the direction of energy and resource flow attributed to microbial predators, adding complexity and nuance to marine food webs.
Funder
Monterey Bay Aquarium Research Institute and GEOMAR
Gordon and Betty Moore Foundation
Monterey Bay Aquarium Research Institute, GEOMAR
GEOMAR
Monterey Bay Aquarium Research Institute
California State University, Monterey Bay
U.S. Department of Energy
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Microbiology (medical),Genetics,Applied Microbiology and Biotechnology,Immunology,Microbiology
Reference138 articles.
1. Worden, A. Z. et al. Rethinking the marine carbon cycle: factoring in the multifarious lifestyles of microbes. Science 347, 1257594 (2015).
2. Bjorbækmo, M. F. M., Evenstad, A., Røsæg, L. L., Krabberød, A. K. & Logares, R. The planktonic protist interactome: where do we stand after a century of research? ISME J. 14, 544–559 (2020).
3. Pandolfi, J. M., Staples, T. L. & Kiessling, W. Increased extinction in the emergence of novel ecological communities. Science 370, 220–222 (2020).
4. Jürgens, K. & Massana, R. in Microbial Ecology of the Oceans (ed. Kirchman, D. L.) 383–441 (John Wiley & Sons, 2008).
5. Archibald, J. M. Endosymbiosis and eukaryotic cell evolution. Curr. Biol. 25, R911–R921 (2015).
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献