Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

Author:

Engmann Anne K.ORCID,Hatch John J.,Nanda Prakruti,Veeraraghavan Priya,Ozkan AbdulkadirORCID,Poulopoulos AlexandrosORCID,Murphy Alexander J.,Macklis Jeffrey D.ORCID

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Reference48 articles.

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2. Menon, S. & Gupton, S. L. Building blocks of functioning brain: cytoskeletal dynamics in neuronal development. Int. Rev. Cell Mol. Biol. 322, 183–245 (2016).

3. Tamariz, E. & Varela-Echavarria, A. The discovery of the growth cone and its influence on the study of axon guidance. Front. Neuroanat. 9, 51 (2015).

4. Vitriol, E. A. & Zheng, J. Q. Growth cone travel in space and time: the cellular ensemble of cytoskeleton, adhesion, and membrane. Neuron 73, 1068–1081 (2012).

5. Ye, X., Qiu, Y., Gao, Y., Wan, D. & Zhu, H. A subtle network mediating axon guidance: intrinsic dynamic structure of growth cone, attractive and repulsive molecular cues, and the intermediate role of signaling pathways. Neural Plast. 2019, 1719829 (2019).

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