Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins-Reference-Cited by-同舟云学术

Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins

Published:2018-11-19 Issue:12 Volume:13 Page:2991-3017
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Elegheert Jonathan^ORCID,Behiels Ester,Bishop Benjamin,Scott Suzanne,Woolley Rachel E.,Griffiths Samuel C.,Byrne Eamon F. X.,Chang Veronica T.,Stuart David I.,Jones E. Yvonne,Siebold Christian,Aricescu A. Radu

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/s41596-018-0075-9.pdf

Reference65 articles.

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2. Chang, V. T. et al. Glycoprotein structural genomics: solving the glycosylation problem. Structure 15, 267–273 (2007).

3. Aricescu, A. R., Lu, W. & Jones, E. Y. A time- and cost-efficient system for high-level protein production in mammalian cells. Acta Crystallogr. D Biol. Crystallogr. 62, 1243–1250 (2006).

4. Chaudhary, S. et al. Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells. Nat. Protoc. 7, 453–466 (2012).

5. Naldini, L., Trono, D. & Verma, I. M. Lentiviral vectors, two decades later. Science 353, 1101–1102 (2016).

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