A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed mouse or cattle embryos-Reference-Cited by-同舟云学术

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A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed mouse or cattle embryos

Published:2020-07-17 Issue:8 Volume:15 Page:2645-2667
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Umehara Takashi,Tsujita Natsumi,Zhu Zhendong,Ikedo Moeka,Shimada Masayuki^ORCID

Funder

Livestock Promotional Funds of Japan Racing Association (JRA, Tokyo, Japan, #H31-138) Hiroshima Cryopreservation Service Co.

China Scholarship Council

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/s41596-020-0348-y.pdf

Reference62 articles.

1. Seidel, G. E. Sexing mammalian sperm – intertwining of commerce, technology, and biology. Anim. Reprod. Sci. 79, 145–156 (2003).

2. Seidel, G. E. Overview of sexing sperm. Theriogenology 68, 443–446 (2007).

3. Naniwa, Y., Sakamoto, Y., Toda, S. & Uchiyama, K. Bovine sperm sex-selection technology in Japan. Reprod. Med. Biol. 18, 17–26 (2019).

4. Ran, F. A. et al. Genome engineering using the CRISPR-Cas9 system. Nat. Protoc. 8, 2281–2308 (2013).

5. Tsien, J. Z. et al. Subregion- and cell type-restricted gene knockout in mouse brain. Cell 87, 1317–1326 (1996).

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