S-acylation of a non-secreted peptide controls plant immunity via secreted-peptide signal activation

Author:

Li WenliangORCID,Ye Tushu,Ye Weixian,Liang Jieyi,Wang WenORCID,Han DanluORCID,Liu XiaoshiORCID,Huang Liting,Ouyang Youwei,Liao Jianwei,Chen Tongsheng,Yang ChengweiORCID,Lai JianbinORCID

Abstract

AbstractSmall peptides modulate multiple processes in plant cells, but their regulation by post-translational modification remains unclear. ROT4 (ROTUNDIFOLIA4) belongs to a family of Arabidopsis non-secreted small peptides, but knowledge on its molecular function and how it is regulated is limited. Here, we find that ROT4 is S-acylated in plant cells. S-acylation is an important form of protein lipidation, yet so far it has not been reported to regulate small peptides in plants. We show that this modification is essential for the plasma membrane association of ROT4. Overexpression of S-acylated ROT4 results in a dramatic increase in immune gene expression. S-acylation of ROT4 enhances its interaction with BSK5 (BRASSINOSTEROID-SIGNALING KINASE 5) to block the association between BSK5 and PEPR1 (PEP RECEPTOR1), a receptor kinase for secreted plant elicitor peptides (PEPs), thereby activating immune signaling. Phenotype analysis indicates that S-acylation is necessary for ROT4 functions in pathogen resistance, PEP response, and the regulation of development. Collectively, our work reveals an important role for S-acylation in the cross-talk of non-secreted and secreted peptide signaling in plant immunity.

Funder

Major Program of Guangdong Basic and Applied Research

National Natural Science Foundation of China

Natural Science Foundation of Guangdong

Guangdong Modern Agro-industry Technology Research System

The Program for Changjiang Scholars

The Guangdong Special Support Program of Young Top-Notch Talent in Science and Technology Innovation

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Molecular Biology,Biochemistry

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