Abstract
AbstractAlpha, beta, and gamma tubulins are essential building blocks for all eukaryotic cells. The functions of the non-canonical tubulins, delta, epsilon, and zeta, however, remain poorly understood and their requirement in mammalian development untested. Herein we have used a spermatogenesis model to define epsilon tubulin (TUBE1) function in mice. We show that TUBE1 is essential for the function of multiple complex microtubule arrays, including the meiotic spindle, axoneme and manchette and in its absence, there is a dramatic loss of germ cells and male sterility. Moreover, we provide evidence for the interplay between TUBE1 and katanin-mediated microtubule severing, and for the sub-specialization of individual katanin paralogs in the regulation of specific microtubule arrays.
Funder
Australian Government Research Training Program Scholarship
National Health and Medical Research Council Ideas Grant
Australian Research Council
National Health and Medical Research Council
Sylvia and Charles Viertel Charitable Foundation
Canadian Institute for Advanced Research (CIFAR) Azrieli Scholarship
State Government of Victoria
Australian Government
Publisher
Springer Science and Business Media LLC
Cited by
2 articles.
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