Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans-Reference-Cited by-同舟云学术

Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans

Published:2008-04 Issue:4 Volume:3 Page:698-709
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Mukhopadhyay Arnab,Deplancke Bart,Walhout Albertha J M,Tissenbaum Heidi A

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2008.38.pdf

Reference27 articles.

1. Walhout, A.J. Unraveling transcription regulatory networks by protein-DNA and protein–protein interaction mapping. Genome Res. 16, 1445–1454 (2006).

2. Collas, P. & Dahl, J.A. Chop it, ChIP it, check it: the current status of chromatin immunoprecipitation. Front. Biosci. 13, 929–943 (2008).

3. Das, P.M., Ramachandran, K., vanWert, J. & Singal, R. Chromatin immunoprecipitation assay. Biotechniques 37, 961–969 (2004).

4. Mukherjee, S. et al. Rapid analysis of the DNA-binding specificities of transcription factors with DNA microarrays. Nat. Genet. 36, 1331–1339 (2004).

5. Meng, X., Brodsky, M.H. & Wolfe, S.A. A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Nat. Biotechnol. 23, 988–994 (2005).

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