Selenium modification of nucleic acids: preparation of oligonucleotides with incorporated 2′-SeMe-uridine for crystallographic phasing of nucleic acid structures-Reference-Cited by-同舟云学术

Selenium modification of nucleic acids: preparation of oligonucleotides with incorporated 2′-SeMe-uridine for crystallographic phasing of nucleic acid structures

Published:2007-03 Issue:3 Volume:2 Page:647-651
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Pallan Pradeep S,Egli Martin

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2007.75.pdf

Reference14 articles.

1. Hendrickson, W.A. Synchrotron crystallography. Trends Biochem. Sci. 25, 637–643 (2001).

2. Doublié, S. Production of selenomethionyl proteins in prokaryotic and eukaryotic expression systems. in Macromolecular Crystallography Protocols: Preparation and Crystallization of Macromolecules Vol. 1 (ed. Doublié, S.) 91–108 (Humana Press, Totowa, New Jersey, 2006).

3. Hendrickson, W.A., Horton, J.R. & LeMaster, D.M. Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): a vehicle for direct determination of three-dimensional structure. EMBO J. 9, 1665–1672 (1990).

4. Du, Q. et al. Internal derivatization of oligonucleotides with selenium for X-ray crystallography using MAD. J. Am. Chem. Soc. 124, 24–25 (2002).

5. Teplova, M. et al. Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwave length anomalous dispersion. Biochimie 84, 849–858 (2002).

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