Competitive PCR–ELISA protocols for the quantitative and the standardized detection of viral genomes-Reference-Cited by-同舟云学术

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Competitive PCR–ELISA protocols for the quantitative and the standardized detection of viral genomes

Published:2007-10 Issue:10 Volume:2 Page:2511-2519
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Musiani Monica,Gallinella Giorgio,Venturoli Simona,Zerbini Marialuisa

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2007.312.pdf

Reference18 articles.

1. Clementi, M., Menzo, S., Manzin, A. & Bagnarelli, P. Quantitative molecular methods in virology. Arch. Virol. 140, 1523–1539 (1995).

2. Gallinella, G. et al. Quantitation of parvovirus B19 DNA sequences by competitive PCR: differential hybridization of the amplicons and immunoenzymatic detection on microplate. Mol. Cell. Probes 11, 127–133 (1997).

3. Zerbini, M. et al. Standardization of a PCR-ELISA assay in serum samples: diagnosis of active parvovirus B19 infection. J. Med. Virol. 59, 239–244 (1999).

4. Fini, F., Gallinella, G., Girotti, S., Zerbini, M. & Musiani, M. Development of a chemiluminescence competitive PCR for the detection and quantification of Parvovirus B19 DNA using a microplate luminometer. Clin. Chem. 45, 1391–1396 (1999).

5. Musiani, M., Venturoli, S., Gallinella, G. & Zerbini, M. Qualitative PCR–ELISA for the detection and typing of viral genomes. Nat. Protoc. 2, 2502–2510 (2007).

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