Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy-Reference-Cited by-同舟云学术

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Investigating macromolecules inside cultured and injected cells by in-cell NMR spectroscopy

Published:2006-12 Issue:6 Volume:1 Page:2701-2709
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Serber Zach,Selenko Philipp,Hänsel Robert,Reckel Sina,Löhr Frank,Ferrell James E,Wagner Gerhard,Dötsch Volker

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2006.181.pdf

Reference19 articles.

1. Fiaux, J., Bertelsen, E.B., Horwich, A.L. & Wuthrich, K. NMR analysis of a 900K GroEL GroES complex. Nature 418, 207–211 (2002).

2. Williams, S.P., Haggie, P.M. & Brindle, K.M. 19F NMR measurements of the rotational mobility of proteins in vivo. Biophys. J. 72, 490–498 (1997).

3. Serber, Z. et al. Methyl groups as probes for proteins and complexes in in-cell NMR experiments. J. Am. Chem. Soc. 126, 7119–7125 (2004).

4. Brindle, K., Williams, S.P. & Boulton, M. 19F NMR detection of a fluorine-labelled enzyme in vivo. FEBS Lett. 255, 121–124 (1989).

5. Serber, Z., Ledwidge, R., Miller, S.M. & Dötsch, V. Evaluation of parameters critical to observing proteins inside living E. coli by in-cell NMR spectroscopy. J. Am. Chem. Soc. 123, 8895–8901 (2001).

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