Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2-Reference-Cited by-同舟云学术

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Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2

Published:2016-02-11 Issue:3 Volume:11 Page:456-475
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Hung Victoria,Udeshi Namrata D,Lam Stephanie S,Loh Ken H,Cox Kurt J,Pedram Kayvon,Carr Steven A,Ting Alice Y

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2016.018.pdf

Reference38 articles.

1. Andersen, J.S. & Mann, M. Organellar proteomics: turning inventories into insights. EMBO Rep. 7, 874–879 (2006).

2. ten Have, S., Boulon, S., Ahmad, Y. & Lamond, A.I. Mass spectrometry-based immuno-precipitation proteomics—the user's guide. Proteomics 11, 1153–1159 (2011).

3. Brunner, Y., Schvartz, D., Couté, Y. & Sanchez, J.-C. Proteomics of regulated secretory organelles. Mass Spectrom. Rev. 28, 844–867 (2009).

4. Rhee, H.-W. et al. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science 339, 1328–1331 (2013).

5. Hung, V. et al. Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging. Mol. Cell 55, 332–341 (2014).

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