An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Biochemistry, Genetics and Molecular Biology
Link
http://www.nature.com/articles/nprot.2007.535.pdf
Reference27 articles.
1. Elbashir, S.M., Lendeckel, W. & Tuschl, T. 'RNA interference is mediated by 21- and 22-nucleotide RNAs'. Genes Dev. 15, 188–200 (2001).
2. Fire, A. et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–811 (1998).
3. Zamore, P.D., Tuschl, T., Sharp, P.A. & Bartel, D.P. 'RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals'. Cell 101, 25–33 (2000).
4. Elbashir, S.M. et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494–498 (2001).
5. Berns, K. et al. A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 428, 431–437 (2004).
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