Removable cranial windows for long-term imaging in awake mice

Author:

Goldey Glenn J,Roumis Demetris K,Glickfeld Lindsey L,Kerlin Aaron M,Reid R Clay,Bonin Vincent,Schafer Dorothy P,Andermann Mark L

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Reference22 articles.

1. Yang, G., Pan, F., Parkhurst, C.N., Grutzendler, J. & Gan, W.-B. Thinned-skull cranial window technique for long-term imaging of the cortex in live mice. Nat. Protoc. 5, 201–208 (2010).

2. Marker, D.F., Tremblay, M.-E., Lu, S.-M., Majewska, A.K. & Gelbard, H.A. A thin-skull window technique for chronic two-photon imaging of murine microglia in models of neuroinflammation. J. Vis. Exp. 43, e2059 (2010).

3. Shih, A.Y., Mateo, C., Drew, P.J., Tsai, P.S. & Kleinfeld, D. A polished and reinforced thinned-skull window for long-term imaging of the mouse brain. J. Vis. Exp. 61, e3742 (2012).

4. Kelly, E.A. & Majewska, A.K. Chronic imaging of mouse visual cortex using a thinned-skull preparation. J. Vis. Exp. 44, e2060 (2010).

5. Dombeck, D.A., Khabbaz, A.N., Collman, F., Adelman, T.L. & Tank, D.W. Imaging large-scale neural activity with cellular resolution in awake, mobile mice. Neuron 56, 43–57 (2007).

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