Determining protein kinase substrate specificity by parallel solution-phase assay of large numbers of peptide substrates
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Biochemistry, Genetics and Molecular Biology
Link
http://www.nature.com/articles/nprot.2006.57.pdf
Reference7 articles.
1. Hutti, J. E. et al. A rapid method for determining protein kinase phosphorylation specificity. Nat. Meth. 1, 27–29 (2004).
2. Songyang, Z. et al. Use of an oriented peptide library to determine the optimal substrates of protein kinases. Curr. Biol. 4, 973–982 (1994).
3. Asensio, C. J. A. and Garcia, R. C. Determination of a large number of kinase activities using peptide substrates, P81 phosphocellulose paper arrays and phosphor imaging. Anal. Biochem. 319, 21–33 (2003).
4. Fujii, K. et al. Kinase peptide specificity: improved determination and relevance to protein phosphorylation. Proc. Natl. Acad. Sci. USA 101, 13744–13749 (2004).
5. Rodriguez, M., Li, S.S., Harper, J.W. & Songyang, Z. An oriented peptide array library (OPAL) strategy to study protein-protein interactions. J. Biol. Chem. 279, 8802–8807 (2004).
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