A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag-Reference-Cited by-同舟云学术

A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag

Published:2007-02 Issue:2 Volume:2 Page:383-391
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Nallamsetty Sreedevi,Waugh David S

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2007.50.pdf

Reference18 articles.

1. Waugh, D.S. Making the most of affinity tags. Trends Biotechnol. 23, 316–320 (2005).

2. Esposito, D. & Chatterjee, D.K. Enhancement of soluble protein expression through the use of fusion tags. Curr. Opin. Biotechnol. 17, 353–358 (2006).

3. Lichty, J.J., Malecki, J.L., Agnew, H.D., Michelson-Horowitz, D.J. & Tan, S. Comparison of affinity tags for protein purification. Protein Expr. Purif. 41, 98–105 (2005).

4. Christendat, D. et al. Structural proteomics: prospects for high throughput sample preparation. Progr. Biophys. Mol. Biol. 73, 339–345 (2000).

5. Davis, G.D., Elisee, C., Newham, D.M. & Harrison, R.G. New fusion protein systems designed to give soluble expression in Escherichia coli . Biotechnol. Bioeng. 65, 382–388 (1999).

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