Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification-Reference-Cited by-同舟云学术

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Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification

Published:2008-08-14 Issue:9 Volume:3 Page:1435-1443
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Braam Stefan R,Denning Chris,Matsa Elena,Young Lorraine E,Passier Robert,Mummery Christine L

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2008.140.pdf

Reference24 articles.

1. Osafune, K. et al. Marked differences in differentiation propensity among human embryonic stem cell lines. Nat. Biotechnol. 26, 313–315 (2008).

2. Giudice, A. & Trounson, A. Genetic modification of human embryonic stem cells for derivation of target cells. Cell Stem Cell 2, 422–433 (2008).

3. Davis, R.P. et al. Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors. Blood 111, 1876–1884 (2008).

4. Urbach, A., Schuldiner, M. & Benvenisty, N. Modeling for Lesch-Nyhan disease by gene targeting in human embryonic stem cells. Stem Cells 22, 635–641 (2004).

5. Costa, M. et al. A method for genetic modification of human embryonic stem cells using electroporation. Nat. Protoc. 2, 792–796 (2007).

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