A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells

Author:

Sonoda Shozo,Spee Christine,Barron Ernesto,Ryan Stephen J,Kannan Ram,Hinton David R

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Reference31 articles.

1. Thumann, G., Hoffmann, S. & Hinton, D.R. Cell biology of the retinal pigment epithelium. In Retina. 4th edn. Vol. 1 (ed. Ryan, S.J.) 137–152 (Elsevier Mosby, Philadelphia, USA, 2006).

2. Rodriguez-Boulan, E. & Nelson, W.J. Morphogenesis of the polarized epithelial cell phenotype. Science 245, 718–725 (1989).

3. Marmor, M.F., Abdul-Rahim, A.S. & Cohen, D.S. The effect of metabolic inhibitors on retinal adhesion and subretinal fluid resorption. Invest. Ophthalmol. Vis. Sci. 19, 893–903 (1980).

4. Miller, S.S., Hughes, B.A. & Machen, T.E. Fluid transport across retinal pigment epithelium is inhibited by cyclic AMP. Proc. Natl Acad. Sci. USA 79, 2111–2115 (1982).

5. Anderson, J.M. & Van Itallie, C.M. Tight junctions and the molecular basis for regulation of paracellular permeability. Am. J. Physiol. 269, G467–G475 (1995).

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