Guidelines for the automated evaluation of Elispot assays-Reference-Cited by-同舟云学术

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Guidelines for the automated evaluation of Elispot assays

Published:2015-06-25 Issue:7 Volume:10 Page:1098-1115
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Janetzki Sylvia,Price Leah,Schroeder Helene,Britten Cedrik M,Welters Marij J P,Hoos Axel

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

https://www.nature.com/articles/nprot.2015.068.pdf

Reference47 articles.

1. Czerkinsky, C.C., Nilsson, L.A., Nygren, H., Ouchterlony, O. & Tarkowski, A. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J. Immunol. Methods 65, 109–121 (1983).

2. Streeck, H., Frahm, N. & Walker, B.D. The role of IFN-γ Elispot assay in HIV vaccine research. Nat. Protoc. 4, 461–469 (2009).

3. Saletti, G., Cuburu, N., Yang, J.S., Dey, A. & Czerkinsky, C. Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood. Nat. Protoc. 8, 1073–1087 (2013).

4. Meier, T., Eulenbruch, H.P., Wrighton-Smith, P., Enders, G. & Regnath, T. Sensitivity of a new commercial enzyme-linked immunospot assay (T SPOT-TB) for diagnosis of tuberculosis in clinical practice. Eur. J. Clin. Microbiol. Infect Dis. 24, 529–536 (2005).

5. Ewer, K.J. et al. Protective CD8+ T-cell immunity to human malaria induced by chimpanzee adenovirus-MVA immunisation. Nat. Commun. 4, 2836 (2013).

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