RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression-Reference-Cited by-同舟云学术

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RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression

Published:2016-06-02 Issue:7 Volume:11 Page:1175-1190
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Wang Hailong,Li Zhen,Jia Ruonan,Hou Yu,Yin Jia,Bian Xiaoying,Li Aiying,Müller Rolf,Stewart A Francis,Fu Jun,Zhang Youming

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2016.054.pdf

Reference46 articles.

1. Van Lanen, S.G. & Shen, B. Microbial genomics for the improvement of natural product discovery. Curr. Opin. Microbiol. 9, 252–260 (2006).

2. Nett, M., Ikeda, H. & Moore, B.S. Genomic basis for natural product biosynthetic diversity in the actinomycetes. Nat. Prod. Rep. 26, 1362–1384 (2009).

3. Lautru, S., Deeth, R.J., Bailey, L.M. & Challis, G.L. Discovery of a new peptide natural product by Streptomyces coelicolor genome mining. Nat. Chem. Biol. 1, 265–269 (2005).

4. Bergmann, S. et al. Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans. Nat. Chem. Biol. 3, 213–217 (2007).

5. Wenzel, S.C. & Müller, R. The impact of genomics on the exploitation of the myxobacterial secondary metabolome. Nat. Prod. Rep. 26, 1385–1407 (2009).

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