Capturing and cultivating single bacterial cells in gel microdroplets to obtain near-complete genomes-Reference-Cited by-同舟云学术

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Capturing and cultivating single bacterial cells in gel microdroplets to obtain near-complete genomes

Published:2014-02-13 Issue:3 Volume:9 Page:608-621
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Dichosa Armand E K,Daughton Ashlynn R,Reitenga Krista G,Fitzsimons Michael S,Han Cliff S

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2014.034.pdf

Reference36 articles.

1. Lasken, R. Single-cell genomic sequencing using multiple displacement amplification. Curr. Opin. Microbiol. 10, 510–516 (2007).

2. Marcy, Y. et al. Dissecting biological 'dark matter' with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth. Proc. Natl. Acad. Sci. USA 104, 11889–11894 (2007).

3. Podar, M. et al. Targeted access to the genomes of low-abundance organisms in complex microbial communities. Appl. Environ. Microbiol. 73, 3205–3214 (2007).

4. Stepanauskas, R. & Sieracki, M.E. Matching phylogeny and metabolism in the uncultured marine bacteria, one cell at a time. Proc. Natl. Acad. Sci. USA 104, 9052–9057 (2007).

5. Rodrigue, S. et al. Whole genome amplification and de novo assembly of single bacterial cells. PLoS ONE 4, e6864 (2009).

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