Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform-Reference-Cited by-同舟云学术

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Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform

Published:2017-12-21 Issue:1 Volume:13 Page:170-194
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Okumus Burak,Baker Charles J,Arias-Castro Juan Carlos,Lai Ghee Chuan,Leoncini Emanuele,Bakshi Somenath,Luro Scott,Landgraf Dirk,Paulsson Johan

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2017.127.pdf

Reference20 articles.

1. Tracy, B.P., Gaida, S.M. & Papoutsakis, E.T. Flow cytometry for bacteria: enabling metabolic engineering, synthetic biology and the elucidation of complex phenotypes. Curr. Opin. Biotechnol. 21, 85–99 (2010).

2. Bongaerts, R.J., Hautefort, I., Sidebotham, J.M. & Hinton, J.C. Green fluorescent protein as a marker for conditional gene expression in bacterial cells. Methods Enzymol. 358, 43–66 (2002).

3. Felip, M., Andreatta, S., Sommaruga, R., Straskrábová, V. & Catalan, J. Suitability of flow cytometry for estimating bacterial biovolume in natural plankton samples: comparison with microscopy data. Appl. Environ. Microbiol. 73, 4508–4514 (2007).

4. Cisse, I.I. et al. Real-time dynamics of RNA polymerase II clustering in live human cells. Science 341, 664–667 (2013).

5. Okumus, B., Yildiz, S. & Toprak, E. Fluidic and microfluidic tools for quantitative systems biology. Curr. Opin. Biotechnol. 25, 30–38 (2014).

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