Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data-Reference-Cited by-同舟云学术

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Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data

Published:2007-08-23 Issue:9 Volume:2 Page:2057-2067
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Hawkins Edwin D,Hommel Mirja,Turner Marian L,Battye Francis L,Markham John F,Hodgkin Philip D

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2007.297.pdf

Reference16 articles.

1. Denizot, F. & Lang, R. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89, 271–277 (1986).

2. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55–63 (1983).

3. Gett, A.V. & Hodgkin, P.D. A cellular calculus for signal integration by T cells. Nat. Immunol. 1, 239–244 (2000).

4. Good, K.L., Bryant, V.L. & Tangye, S.G. Kinetics of human B cell behavior and amplification of proliferative responses following stimulation with IL-21. J. Immunol. 177, 5236–5247 (2006).

5. Deenick, E.K., Gett, A.V. & Hodgkin, P.D. Stochastic model of T cell proliferation: a calculus revealing IL-2 regulation of precursor frequencies, cell cycle time, and survival. J. Immunol. 170, 4963–4972 (2003).

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