Touchdown PCR for increased specificity and sensitivity in PCR amplification
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Biochemistry, Genetics and Molecular Biology
Link
http://www.nature.com/articles/nprot.2008.133.pdf
Reference40 articles.
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3. Mullis, K. et al. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb. Symp. Quant. Biol. 51 (Part 1): 263–273 (1986).
4. Kolmodin, L.A. & Birch, D.E. Polymerase chain reaction. Basic principles and routine practice. Methods Mol. Biol. 192, 3–18 (2002).
5. Kramer, M.F. & Coen, D.M. Enzymatic amplification of DNA by PCR: standard procedures and optimization. Curr. Protoc. Mol. Biol. Chapter 15, Unit 15 1 (2001).
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