The energy landscape for R-loop formation by the CRISPR–Cas Cascade complex-Reference-Cited by-同舟云学术

The energy landscape for R-loop formation by the CRISPR–Cas Cascade complex

Published:2023-07 Issue:7 Volume:30 Page:1040-1047
ISSN:1545-9993
Container-title:Nature Structural & Molecular Biology
language:en
Short-container-title:Nat Struct Mol Biol

Author:

Kauert Dominik J.^ORCID,Madariaga-Marcos Julene,Rutkauskas Marius,Wulfken Alexander,Songailiene Inga^ORCID,Sinkunas Tomas,Siksnys Virginijus^ORCID,Seidel Ralf^ORCID

Publisher

Springer Science and Business Media LLC

Subject

Molecular Biology,Structural Biology

Link

https://www.nature.com/articles/s41594-023-01019-2.pdf

Reference46 articles.

1. Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V. Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc. Natl Acad. Sci. USA 109, E2579–E2586 (2012).

2. Brouns, S. J. J. et al. Small CRISPR RNAs guide antiviral defense in prokaryotes. Science 321, 960–964 (2008).

3. Jore, M. M. et al. Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Nat. Struct. Mol. Biol. 18, 529–536 (2011).

4. Xiao, Y. et al. Structure basis for directional R-loop formation and substrate handover mechanisms in type I CRISPR–Cas system. Cell 170, 48–60.e11 (2017).

5. Szczelkun, M. D. et al. Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes. Proc. Natl Acad. Sci. USA 111, 9798–9803 (2014).

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