Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments
Author:
Publisher
Springer Science and Business Media LLC
Subject
Multidisciplinary
Link
http://www.nature.com/articles/srep10655.pdf
Reference28 articles.
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2. Gibson, D. G. et al. Complete chemical synthesis, assembly and cloning of a Mycoplasma genitalium genome. Science 319, 1215–1220 (2008).
3. Gibson, D. G. et al. Creation of a bacterial cell controlled by a chemically synthesized genome. Science 329, 52–56 (2010).
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5. Tsuge, K., Matsui, K. & Itaya, M. One step assembly of multiple DNA fragments with a designed order and orientation in Bacillus subtilis plasmid. Nucleic Acids Res. 31, e133 (2003).
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