Abstract
Abstract
Several methods have been proposed for separation of eimerian oocysts and trichostrongylid eggs from extraneous debris; however, these methods have been considered to be still inconvenient in terms of time and wide-ranging applications. We describe herein an alternative way using the combination of electrical cream separator and vacuum filtration for harvesting and purifying eimerian oocysts and haemonchine eggs on large-scale applications with approximately 81% and 92% recovery rates for oocysts and nematode eggs obtained from avian and ovine faeces, correspondingly. The sporulation percentages as a measure of viability in the harvested oocysts and eggs from dry faecal materials are nearly 68% and 74%, respectively, and 12 liters of faecal suspension can be processed in approximately 7.5 min. The mode of separation in terms of costs (i.e. simple laboratory equipments and comparably cheap reagents) and benefits renders the reported procedure an appropriate pursuit to harvest and purify parasite oocysts and eggs on a large scale in the shortest duration from diverse volumes of environmental samples compared to the modified traditional sucrose gradient, which can be employed on a small scale.
Publisher
Springer Science and Business Media LLC
Reference33 articles.
1. Davis, L. R. & Bowman, G. W. The endogenous development of Eimeria zurnii, a pathogenic coccidium of cattle. American journal of veterinary research 18, 569–574 (1957).
2. Grafner, G. & Graubmann, H. D. Remarks on the pathogenicity of Eimeria species exemplified by cattle coccidiosis. Angewandte Parasitologie 20, 202–209 (1979).
3. Niilo, L. The effect of dexamethasone on bovine coccidiosis. Canadian journal of comparative medicine: Revue canadienne de medecine comparee 34, 325–328 (1970).
4. Shirley, M. W., Smith, A. L. & Tomley, F. M. The biology of avian Eimeria with an emphasis on their control by vaccination. Advances in parasitology 60, 285–330, doi: 10.1016/s0065-308x(05)60005-x (2005).
5. O’Connor, L. J., Walkden-Brown, S. W. & Kahn, L. P. Ecology of the free-living stages of major trichostrongylid parasites of sheep. Veterinary parasitology 142, 1–15, doi: 10.1016/j.vetpar.2006.08.035 (2006).
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