Abstract
AbstractNatural gas seeps release significant amounts of methane and other gases including ethane and propane contributing to global climate change. In this study, bacterial actively consuming short-chain alkanes were identified by cultivation, whole-genome sequencing, and stable-isotope probing (SIP)-metagenomics using 13C-propane and 13C-ethane from two different natural gas seeps, Pipe Creek and Andreiasu Everlasting Fire. Nearly 100 metagenome-assembled genomes (MAGs) (completeness 70–99%) were recovered from both sites. Among these, 16 MAGs had genes encoding the soluble di-iron monooxygenase (SDIMO). The MAGs were affiliated to Actinobacteria (two MAGs), Alphaproteobacteria (ten MAGs), and Gammaproteobacteria (four MAGs). Additionally, three gaseous-alkane degraders were isolated in pure culture, all of which could grow on ethane, propane, and butane and possessed SDIMO-related genes. Two Rhodoblastus strains (PC2 and PC3) were from Pipe Creek and a Mycolicibacterium strain (ANDR5) from Andreiasu. Strains PC2 and PC3 encoded putative butane monooxygenases (MOs) and strain ANDR5 contained a propane MO. Mycolicibacterium strain ANDR5 and MAG19a, highly abundant in incubations with 13C-ethane, share an amino acid identity (AAI) of 99.3%. We show using a combination of enrichment and isolation, and cultivation-independent techniques, that these natural gas seeps contain a diverse community of active bacteria oxidising gaseous-alkanes, which play an important role in biogeochemical cycling of natural gas.
Publisher
Springer Science and Business Media LLC
Subject
Ecology, Evolution, Behavior and Systematics,Microbiology
Reference76 articles.
1. Saunois M, Stavert AR, Poulter B, Bousquet P, Canadell JG, Jackson RB, et al. The global methane budget 2000–2017. Earth Syst Sci Data. 2020;12:1561–623.
2. Myhre G, Shindell D, Bréon F-M, Collins W, Fuglestvedt J, Huang J, et al. “Anthropogenic and natural radiative forcing”. In: Stocker TF, et al. Eds. Climate Change 2013: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge, UK: Cambridge University Press; 2013. chap. 8
3. Reddy CM, Arey JS, Seewald JS, Sylva SP, Lemkau KL, Nelson RK, et al. Composition and fate of gas and oil released to the water column during the Deepwater Horizon oil spill. Proc Natl Acad Sci USA. 2012;109:20229–34.
4. Etiope G, Schwietzke S. Global geological methane emissions: an update of top-down and bottom-up estimates. Elementa. 2019;7:47.
5. Mazzini A, Sciarra A, Etiope G, Sadavarte P, Houweling S, Pandey S, et al. Relevant methane emission to the atmosphere from a geological gas manifestation. Sci Rep. 2021;11:4138.
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献