Extensive proteome and functional genomic profiling of variability between genetically identical human B-lymphoblastoid cells

Author:

Laczik Miklós,Erdős Edina,Ozgyin Lilla,Hevessy Zsuzsanna,Csősz ÉvaORCID,Kalló Gergő,Nagy TiborORCID,Barta Endre,Póliska Szilárd,Szatmári IstvánORCID,Bálint Bálint LászlóORCID

Abstract

AbstractIn life-science research isogenic B-lymphoblastoid cell lines (LCLs) are widely known and preferred for their genetic stability – they are often used for studying mutations for example, where genetic stability is crucial. We have shown previously that phenotypic variability can be observed in isogenic B-lymphoblastoid cell lines. Isogenic LCLs present well-defined phenotypic differences on various levels, for example on the gene expression level or the chromatin level. Based on our investigations, the phenotypic variability of the isogenic LCLs is accompanied by certain genetic variation too. We have developed a compendium of LCL datasets that present the phenotypic and genetic variability of five isogenic LCLs from a multiomic perspective. In this paper, we present additional datasets generated with Next Generation Sequencing techniques to provide genomic and transcriptomic profiles (WGS, RNA-seq, single cell RNA-seq), protein-DNA interactions (ChIP-seq), together with mass spectrometry and flow cytometry datasets to monitor the changes in the proteome. We are sharing these datasets with the scientific community according to the FAIR principles for further investigations.

Publisher

Springer Science and Business Media LLC

Subject

Library and Information Sciences,Statistics, Probability and Uncertainty,Computer Science Applications,Education,Information Systems,Statistics and Probability

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