Abstract
AbstractThe yellow peach moth, Conogethes punctiferalis, is a highly polyphagous pest widespread in eastern and southern Asia. It demonstrates a unique ability to adapt to rotten host fruits and displays resistance to pathogenic microorganisms, including fungi. However, the lack of available genomic resources presents a challenge in comprehensively understanding the evolution of its innate immune genes. Here, we report a high-quality chromosome-level reference genome for C. punctiferalis utilizing PacBio HiFi sequencing and Hi-C technology. The genome assembly was 494 Mb in length with a contig N50 of 3.25 Mb. We successfully anchored 1,226 contigs to 31 pseudochromosomes. Our BUSCO analysis further demonstrated a gene coverage completeness of 96.3% in the genome assembly. Approximately 43% repeat sequences and 21,663 protein-coding genes were identified. In addition, we resequenced 110 C. punctiferalis individuals from east China, achieving an average coverage of 18.4 × and identifying 5.8 million high-quality SNPs. This work provides a crucial resource for understanding the evolutionary mechanism of C. punctiferalis’ innate immune system and will help in developing new antibacterial drugs.
Publisher
Springer Science and Business Media LLC
Subject
Library and Information Sciences,Statistics, Probability and Uncertainty,Computer Science Applications,Education,Information Systems,Statistics and Probability
Reference43 articles.
1. Shwe, S. M., Prabu, S., Jing, D., He, K. & Wang, Z. Synergistic interaction of Cry1Ah and Vip3Aa19 proteins combination with midgut ATP-binding cassette subfamily C receptors of Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae). Int J Biol Macromol. 213, 871–879 (2022).
2. He, K. et al. Evaluation of transgenic Bt corn for resistance to the Asian corn borer (Lepidoptera: Pyralidae). J Econ Entomol. 96, 935–940 (2003).
3. Yang, S. et al. Impact of durian fruit borer Conogethes punctiferalis on yield loss of summer corn by injuring corn ears. J Plant Prot. 42, 991–996 (2015).
4. Lu, J., Wang, Z., He, K. & Liu, Y. Research history, progresses and prospects in the yellow peach moth, Conogethes punctiferalis. Plant Prot. 36, 31–38 (2010).
5. Guo, H. et al. Penicillium fungi mediate behavioral responses of the yellow peach moth, Conogethes punctiferalis (Guenée) to apple fruits via altering the emissions of host plant VOCs. Arch Insect Biochem Physiol. 110, e21895 (2022).