In vivo and in vitro protein solubility assays using split GFP
Author:
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology,Biochemistry,Biotechnology
Link
http://www.nature.com/articles/nmeth932.pdf
Reference9 articles.
1. Knaust, R.K. & Nordlund, P. Screening for soluble expression of recombinant proteins in a 96-well format. Anal. Biochem. 297, 79–85 (2001).
2. Vincentelli, R., Canaan, S., Offant, J., Cambillau, C. & Bignon, C. Automated expression and solubility screening of His-tagged proteins in 96-well format. Anal. Biochem. 346, 77–84 (2005).
3. Nguyen, H., Martinez, B., Oganesyan, N. & Kim, R. An automated small-scale protein expression and purification screening provides beneficial information for protein production. J. Struct. Funct. Genomics 5, 23–27 (2004).
4. Wigley, W.C., Stidham, R.D., Smith, N.M., Hunt, J.F. & Thomas, P.J. Protein solubility and folding monitored in vivo by structural complementation of a genetic marker protein. Nat. Biotechnol. 19, 131–136 (2001).
5. Horecka, J. et al. Antibody-free method for protein detection on blots using enzyme fragment complementation. Biotechniques 40, 381–383 (2006).
Cited by 244 articles. 订阅此论文施引文献 订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
1. A modular system to label endogenous presynaptic proteins using split fluorophores inC. elegans;2024-07-30
2. Comprehensive Review on Bimolecular Fluorescence Complementation and Its Application in Deciphering Protein–Protein Interactions in Cell Signaling Pathways;Biomolecules;2024-07-17
3. A pyruvate transporter in the apicoplast of apicomplexan parasites;Proceedings of the National Academy of Sciences;2024-06-12
4. Screening signal peptidase based on split-GFP assembly technology to promote the secretion of alkaline protease AprE in Bacillus amyloliquefaciens;International Journal of Biological Macromolecules;2024-06
5. Experimental and computational approaches for membrane protein insertion and topology determination;Methods;2024-06
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线，采集、加工和组织学术论文而形成的新型学术文献查询和分析系统，可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容，当前同舟云学术共收录了国内外主流学术期刊6万余种，收集的期刊论文及会议论文总量共计约1.5亿篇，并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询！咨询电话：010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3