Abstract
AbstractJujube (Ziziphus jujuba Mill.) is an important perennial fruit tree with a range of interesting horticultural traits. It was domesticated from wild jujube (Ziziphus acidojujuba), but the genomic variation dynamics and genetic changes underlying its horticultural traits during domestication are poorly understood. Here, we report a comprehensive genome variation map based on the resequencing of 350 accessions, including wild, semi-wild and cultivated jujube plants, at a >15× depth. Using the combination of a genome-wide association study (GWAS) and selective sweep analysis, we identified several candidate genes potentially involved in regulating seven domestication traits in jujube. For fruit shape and kernel shape, we integrated the GWAS approach with transcriptome profiling data, expression analysis and the transgenic validation of a candidate gene to identify a causal gene, ZjFS3, which encodes an ethylene-responsive transcription factor. Similarly, we identified a candidate gene for bearing-shoot length and the number of leaves per bearing shoot and two candidate genes for the seed-setting rate using GWAS. In the selective sweep analysis, we also discovered several putative genes for the presence of prickles on bearing shoots and the postharvest shelf life of fleshy fruits. This study outlines the genetic basis of jujube domestication and evolution and provides a rich genomic resource for mining other horticulturally important genes in jujube.
Funder
Science and Technology Department of Henan Province
Education Department of Henan Province
Publisher
Oxford University Press (OUP)
Subject
Horticulture,Plant Science,Genetics,Biochemistry,Biotechnology
Reference57 articles.
1. Qu, Z. & Wang, Y. Chinese Fruit Trees Record-Chinese Jujube (China Forestry Publishing House, 1993).
2. Liu, M., Liu, P. & Liu, G. Advances of research on germplasm resources of Chinese jujube. Acta Hortic. 993, 15–20 (2013).
3. Liu, M. J. Chinese jujube: botany and horticulture. Hort. Revi 32, 229–298 (2006).
4. Huang, J. et al. The jujube genome provides insights into genome evolution and the domestication of sweetness/acidity taste in fruit trees. PLoS Genet. 12, e1006433 (2016).
5. Wang, S. et al. The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality. Nat. Genet. 47, 949–954 (2015).
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