Abstract
AbstractStudies have shown that melatonin regulates the expression of various elements in the biosynthesis and catabolism of plant hormones. In contrast, the effects of these different plant hormones on the biosynthesis and metabolism of melatonin and their underlying molecular mechanisms are still unclear. In this study, the melatonin biosynthesis pathway was proposed from constructed metabolomic and transcriptomic libraries from hickory (Carya cathayensis Sarg.) nuts. The candidate pathway genes were further identified by phylogenetic analysis, amino-acid sequence alignment, and subcellular localization. Notably, most of the transcription factor-related genes coexpressed with melatonin pathway genes were hormone-responsive genes. Furthermore, dual-luciferase and yeast one‐hybrid assays revealed that CcEIN3 (response to ethylene) and CcAZF2 (response to abscisic acid) could activate melatonin biosynthesis pathway genes, a tryptophan decarboxylase coding gene (CcTDC1) and an N-acetylserotonin methyltransferase coding gene (CcASMT1), by directly binding to their promoters, respectively. Our results provide a molecular basis for the characterization of novel melatonin biosynthesis regulatory mechanisms and demonstrate for the first time that abscisic acid and ethylene can regulate melatonin biosynthesis.
Publisher
Oxford University Press (OUP)
Subject
Horticulture,Plant Science,Genetics,Biochemistry,Biotechnology
Reference67 articles.
1. Reiter, R. J. et al. Melatonin as a mitochondria-targeted antioxidant: one of evolution’s best ideas. Cell. Mol. Life Sci. 74, 3863–3881 (2017).
2. Aaron, B. L., James, D. C., Yoshiyata, T., Teh, H. L. & Wataru, M. Isolation of melatonin, a pineal factor that lightens melanocytes. J. Am. Chem. Soc. 80, 2587 (1958).
3. Dubbels, R. et al. Melatonin in edible plants identified by radioimmunoassay and by high-performance liquid chromatography-mass spectrometry. J. Pineal Res. 18, 28–31 (1995).
4. Arnao, M. B. & Hernandez-Ruiz, J. Functions of melatonin in plants: a review. J. Pineal Res. 59, 133–150 (2015).
5. Nawaz, M. A. et al. Melatonin: current status and future perspectives in plant science. Front. Plant Sci. 6, 1230 (2015).
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