A postnatal network of co-hepato/pancreatic stem/progenitors in the biliary trees of pigs and humans
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Published:2023-08-01
Issue:1
Volume:8
Page:
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ISSN:2057-3995
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Container-title:npj Regenerative Medicine
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language:en
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Short-container-title:npj Regen Med
Author:
Zhang WenchengORCID, Wang XichengORCID, Lanzoni Giacomo, Wauthier Eliane, Simpson Sean, Ezzell Jennifer Ashley, Allen Amanda, Suitt Carolyn, Krolik Jonah, Jhirad Alexander, Dominguez-Bendala JuanORCID, Cardinale Vincenzo, Alvaro Domenico, Overi Diletta, Gaudio Eugenio, Sethupathy PraveenORCID, Carpino GuidoORCID, Adin ChristopherORCID, Piedrahita Jorge A, Mathews Kyle, He Zhiying, Reid Lola McAdamsORCID
Abstract
AbstractA network of co-hepato/pancreatic stem/progenitors exists in pigs and humans in Brunner’s Glands in the submucosa of the duodenum, in peribiliary glands (PBGs) of intrahepatic and extrahepatic biliary trees, and in pancreatic duct glands (PDGs) of intrapancreatic biliary trees, collectively supporting hepatic and pancreatic regeneration postnatally. The network is found in humans postnatally throughout life and, so far, has been demonstrated in pigs postnatally at least through to young adulthood. These stem/progenitors in vivo in pigs are in highest numbers in Brunner’s Glands and in PDGs nearest the duodenum, and in humans are in Brunner’s Glands and in PBGs in the hepato/pancreatic common duct, a duct missing postnatally in pigs. Elsewhere in PDGs in pigs and in all PDGs in humans are only committed unipotent or bipotent progenitors. Stem/progenitors have genetic signatures in liver/pancreas-related RNA-seq data based on correlation, hierarchical clustering, differential gene expression and principal component analyses (PCA). Gene expression includes representative traits of pluripotency genes (SOX2, OCT4), endodermal transcription factors (e.g. SOX9, SOX17, PDX1), other stem cell traits (e.g. NCAM, CD44, sodium iodide symporter or NIS), and proliferation biomarkers (Ki67). Hepato/pancreatic multipotentiality was demonstrated by the stem/progenitors’ responses under distinct ex vivo conditions or in vivo when patch grafted as organoids onto the liver versus the pancreas. Therefore, pigs are logical hosts for translational/preclinical studies for cell therapies with these stem/progenitors for hepatic and pancreatic dysfunctions.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Developmental Biology,Biomedical Engineering,Medicine (miscellaneous)
Reference81 articles.
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