Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform-Reference-Cited by-同舟云学术

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Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

Published:2018-03-12 Issue:1 Volume:9 Page:
ISSN:2041-1723
Container-title:Nature Communications
language:en
Short-container-title:Nat Commun

Author:

Ma Fuqiang,Chung Meng Ting,Yao Yuan,Nidetz Robert,Lee Lap Man,Liu Allen P.,Feng Yan,Kurabayashi Katsuo,Yang Guang-Yu

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

Link

http://www.nature.com/articles/s41467-018-03492-6.pdf

Reference47 articles.

1. Arnold, F. H. Design by directed evolution. Acc. Chem. Res. 31, 125–131 (1998).

2. Reetz, M. T. Application of directed evolution in the development of enantioselective enzymes. Pure Appl. Chem. 72, 1615–1622 (2000).

3. Otten, L. G., Hollmann, F. & Arends, I. W. Enzyme engineering for enantioselectivity: from trial-and-error to rational design? Trends Biotechnol. 28, 46–54 (2010).

4. Acevedo-Rocha, C. G., Agudo, R. & Reetz, M. T. Directed evolution of stereoselective enzymes based on genetic selection as opposed to screening systems. J. Biotechnol. 191, 3–10 (2014).

5. Denard, C. A., Ren, H. & Zhao, H. Improving and repurposing biocatalysts via directed evolution. Curr. Opin. Chem. Biol. 25, 55–64 (2015).

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