The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant

Author:

Bogdanow Boris,Wang XiORCID,Eichelbaum Katrin,Sadewasser AnneORCID,Husic Immanuel,Paki Katharina,Budt Matthias,Hergeselle Martha,Vetter Barbara,Hou Jingyi,Chen WeiORCID,Wiebusch LüderORCID,Meyer Irmtraud M.,Wolff ThorstenORCID,Selbach MatthiasORCID

Abstract

AbstractPandemic influenza A virus (IAV) outbreaks occur when strains from animal reservoirs acquire the ability to infect and spread among humans. The molecular basis of this species barrier is incompletely understood. Here we combine metabolic pulse labeling and quantitative proteomics to monitor protein synthesis upon infection of human cells with a human- and a bird-adapted IAV strain and observe striking differences in viral protein synthesis. Most importantly, the matrix protein M1 is inefficiently produced by the bird-adapted strain. We show that impaired production of M1 from bird-adapted strains is caused by increased splicing of the M segment RNA to alternative isoforms. Strain-specific M segment splicing is controlled by the 3′ splice site and functionally important for permissive infection.In silicoand biochemical evidence shows that avian-adapted M segments have evolved different conserved RNA structure features than human-adapted sequences. Thus, we identify M segment RNA splicing as a viral host range determinant.

Funder

Bundesministerium für Bildung und Forschung

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

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