Abstract
AbstractReceptor-mediated transport of soluble proteins is nature’s key to empowering eukaryotic cells to access a plethora of macromolecules, either by direct accumulation or as products from resulting biochemical pathways. The transport efficiency of these mechanisms results from the receptor’s capability to capture, transport, and release ligands on the one hand and the cycling ability that allows for performing multiple rounds of ligand transport on the other. However, the plant VACUOLAR SORTING RECEPTOR (VSR) protein family is diverse, and their ligand-specificity and bidirectional trafficking routes and transport mechanisms remain highly controversial. Here we employ nanobody-epitope interaction-based molecular tools to assess the function of the VSR 7 in vivo. We demonstrate the specificity of the VSR7 for sequence-specific vacuolar sorting signals, and we trace its anterograde transport and retrograde recycling route. VSR7 localizes at the cis-Golgi apparatus at steady state conditions and transports ligands downstream to release them in the trans-Golgi network/early endosome (TGN/EE) before undergoing clathrin-dependent recycling from the TGN/EE back to the cis-Golgi.
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
Reference55 articles.
1. Rohrer, J. & Kornfeld, R. Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network. Mol. Biol. Cell 12, 1623–1631 (2001).
2. Gonzalez-Noriega, A., Grubb, J. H., Talkad, V. & Sly, W. S. Chloroquine inhibits lysosomal enzyme pinocytosis and enhances lysosomal enzyme secretion by impairing receptor recycling. J. Cell Biol. 85, 839–852 (1980).
3. Niemes, S. et al. Sorting of plant vacuolar proteins is initiated in the ER. Plant J. 62, 601–614 (2010).
4. Holwerda, B. C., Padgett, H. S. & Rogers, J. C. Proaleurain vacuolar targeting is mediated by short contiguous peptide interactions. Plant Cell 4, 307–318 (1992).
5. Matsuoka, K. & Nakamura, K. Propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting. Proc. Natl Acad. Sci. USA 88, 834–838 (1991).
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献