Phosphoribosylpyrophosphate synthetase as a metabolic valve advances Methylobacterium/Methylorubrum phyllosphere colonization and plant growth

Author:

Zhang Cong,Zhou Di-Fei,Wang Meng-Ying,Song Ya-Zhen,Zhang Chong,Zhang Ming-Ming,Sun Jing,Yao Lu,Mo Xu-Hua,Ma Zeng-Xin,Yuan Xiao-Jie,Shao Yi,Wang Hao-Ran,Dong Si-Han,Bao Kai,Lu Shu-Huan,Sadilek Martin,Kalyuzhnaya Marina G.ORCID,Xing Xin-Hui,Yang SongORCID

Abstract

AbstractThe proficiency of phyllosphere microbiomes in efficiently utilizing plant-provided nutrients is pivotal for their successful colonization of plants. The methylotrophic capabilities of Methylobacterium/Methylorubrum play a crucial role in this process. However, the precise mechanisms facilitating efficient colonization remain elusive. In the present study, we investigate the significance of methanol assimilation in shaping the success of mutualistic relationships between methylotrophs and plants. A set of strains originating from Methylorubrum extorquens AM1 are subjected to evolutionary pressures to thrive under low methanol conditions. A mutation in the phosphoribosylpyrophosphate synthetase gene is identified, which converts it into a metabolic valve. This valve redirects limited C1-carbon resources towards the synthesis of biomass by up-regulating a non-essential phosphoketolase pathway. These newly acquired bacterial traits demonstrate superior colonization capabilities, even at low abundance, leading to increased growth of inoculated plants. This function is prevalent in Methylobacterium/Methylorubrum strains. In summary, our findings offer insights that could guide the selection of Methylobacterium/Methylorubrum strains for advantageous agricultural applications.

Funder

National Natural Science Foundation of China

the U.S. Department of Energy under the DOE Office of Science (SC) RENEW contract

National Key Research and Development Program of China

Publisher

Springer Science and Business Media LLC

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