Abstract
AbstractThe cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity is regulated in the blebbing cells; the cytoplasm of rapidly expanding membrane blebs is more disordered than the cytoplasm of retracting blebs. The increase of cytoplasmic fluidity in the expanding bleb is caused by a sharp rise in the calcium concentration. The STIM-Orai1 pathway regulates this rapid and restricted increase of calcium in the expanding blebs. Conversely, activated ERM protein binds to Orai1 to inhibit the store-operated calcium entry in retracting blebs, which results in decreased in cytoplasmic calcium, rapid reassembly of the actin cortex.
Funder
MEXT | Japan Society for the Promotion of Science
MEXT | JST | Precursory Research for Embryonic Science and Technology
Japan Agency for Medical Research and Development
Uehara Memorial Foundation
Takeda Science Foundation
Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering
Merck & Co., Inc. | MSD K.K.
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
Cited by
28 articles.
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