Abstract
Abstract
Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.
Funder
European Molecular Biology Laboratory EMBL International PhD Programme
École Polytechnique Fédérale de Lausanne the NCCR Chemical Biology
Max Planck Society École Polytechnique Fédérale de Lausanne a grant from the Swiss Commission for Technology and Innovation (CTI) the NCCR Chemical Biology
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
Cited by
61 articles.
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