High-throughput small molecule screen identifies inhibitors of microsporidia invasion and proliferation in C. elegans

Author:

Murareanu Brandon M.,Antao Noelle V.,Zhao WinnieORCID,Dubuffet Aurore,El Alaoui Hicham,Knox JessicaORCID,Ekiert Damian C.ORCID,Bhabha Gira,Roy Peter J.ORCID,Reinke Aaron W.ORCID

Abstract

AbstractMicrosporidia are a diverse group of fungal-related obligate intracellular parasites that infect most animal phyla. Despite the emerging threat that microsporidia represent to humans and agricultural animals, few reliable treatment options exist. Here, we develop a high-throughput screening method for the identification of chemical inhibitors of microsporidia infection, using liquid cultures of Caenorhabditis elegans infected with the microsporidia species Nematocida parisii. We screen a collection of 2560 FDA-approved compounds and natural products, and identify 11 candidate microsporidia inhibitors. Five compounds prevent microsporidia infection by inhibiting spore firing, whereas one compound, dexrazoxane, slows infection progression. The compounds have in vitro activity against several other microsporidia species, including those known to infect humans. Together, our results highlight the effectiveness of C. elegans as a model host for drug discovery against intracellular pathogens, and provide a scalable high-throughput system for the identification and characterization of microsporidia inhibitors.

Funder

U.S. Department of Health & Human Services | NIH | Office of Extramural Research, National Institutes of Health

Pew Charitable Trusts

Gouvernement du Canada | Canadian Institutes of Health Research

Alfred P. Sloan Foundation

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary

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