A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies

Author:

Yao Zhong,Drecun Luka,Aboualizadeh Farzaneh,Kim Sun Jin,Li ZhijieORCID,Wood Heidi,Valcourt Emelissa J.,Manguiat Kathy,Plenderleith Simon,Yip LilyORCID,Li Xinliu,Zhong Zoe,Yue Feng Yun,Closas Tatiana,Snider Jamie,Tomic Jelena,Drews Steven J.,Drebot Michael A.,McGeer Allison,Ostrowski Mario,Mubareka Samira,Rini James M.,Owen ShawnORCID,Stagljar IgorORCID

Abstract

AbstractBetter diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, to meet this urgent demand, we report a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on a tri-part Nanoluciferase (tNLuc) approach, in which the spike protein of SARS-CoV-2 and protein G, fused respectively to two different tNLuc tags, are used as antibody probes. Target engagement of the probes allows reconstitution of a functional luciferase in the presence of the third tNLuc component. The assay is performed directly in the liquid phase of patient sera and enables rapid, quantitative and low-cost detection. We show that SATiN has a similar sensitivity to ELISA, and its readouts are consistent with various neutralizing antibody assays. This proof-of-principle study suggests potential applications in diagnostics, as well as disease and vaccination management.

Funder

Connaught Fund

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

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