Abstract
AbstractBetter diagnostic tools are needed to combat the ongoing COVID-19 pandemic. Here, to meet this urgent demand, we report a homogeneous immunoassay to detect IgG antibodies against SARS-CoV-2. This serological assay, called SATiN, is based on a tri-part Nanoluciferase (tNLuc) approach, in which the spike protein of SARS-CoV-2 and protein G, fused respectively to two different tNLuc tags, are used as antibody probes. Target engagement of the probes allows reconstitution of a functional luciferase in the presence of the third tNLuc component. The assay is performed directly in the liquid phase of patient sera and enables rapid, quantitative and low-cost detection. We show that SATiN has a similar sensitivity to ELISA, and its readouts are consistent with various neutralizing antibody assays. This proof-of-principle study suggests potential applications in diagnostics, as well as disease and vaccination management.
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
Reference43 articles.
1. Weissleder, R., Lee, H., Ko, J. & Pittet, M. J. COVID-19 diagnostics in context. Sci. Transl. Med. 12, eabc1931 (2020).
2. Tang, Y., Schmitz, J. E., Persing, D. H. & Stratton, C. W. Laboratory diagnosis of COVID-19: current issues and challenges. J. Clin. Microbiol. 58, e00512–e00520 (2020).
3. Peeling, R. W. et al. Serology testing in the COVID-19 pandemic response. Lancet Infect. Dis. 20, e245–e249 (2020).
4. Huang, A. T. et al. A systematic review of antibody mediated immunity to coronaviruses: kinetics, correlates of protection, and association with severity. Nat. Commun. 11, 4704 (2020).
5. Lisboa Bastos, M. et al. Diagnostic accuracy of serological tests for covid-19: systematic review and meta-analysis. BMJ 370, m2516 (2020).
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