Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis-Reference-Cited by-同舟云学术

Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis

Published:2020-07-07 Issue:1 Volume:11 Page:
ISSN:2041-1723
Container-title:Nature Communications
language:en
Short-container-title:Nat Commun

Author:

Tan Yong Zi^ORCID,Rodrigues José^ORCID,Keener James E.,Zheng Ruixiang Blake,Brunton Richard,Kloss Brian^ORCID,Giacometti Sabrina I.,Rosário Ana L.,Zhang Lei,Niederweis Michael^ORCID,Clarke Oliver B.^ORCID,Lowary Todd L.^ORCID,Marty Michael T.^ORCID,Archer Margarida^ORCID,Potter Clinton S.,Carragher Bridget^ORCID,Mancia Filippo

Abstract

AbstractArabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 Å resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

Link

http://www.nature.com/articles/s41467-020-17202-8.pdf

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