Abstract
AbstractInvasive micropapillary carcinoma (IMPC) has very high rates of lymphovascular invasion and lymph node metastasis and has been reported in several organs. However, the genomic mechanisms underlying its metastasis are unclear. Here, we perform whole-genome sequencing of tumor cell clusters from primary IMPC and paired axillary lymph node metastases. Cell clusters in multiple lymph node foci arise from a single subclone of the primary tumor. We find evidence that the monoclonal metastatic ancestor in primary IMPC shares high frequency copy-number loss of PRDM16 and IGSF9 and the copy number gain of ALDH2. Immunohistochemistry analysis further shows that low expression of IGSF9 and PRDM16 and high expression of ALDH2 are associated with lymph node metastasis and poor survival of patients with IMPC. We expect these genomic and evolutionary profiles to contribute to the accurate diagnosis of IMPC.
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
Reference65 articles.
1. Fisher, E. R., Palekar, A. S., Redmond, C., Barton, B. & Fisher, B. Pathologic findings from the National Surgical Adjuvant Breast Project (protocol no. 4). VI. Invasive papillary cancer. Am. J. Clin. Pathol. 73, 313–322 (1980).
2. Siriaunkgul, S. & Tavassoli, F. A. Invasive micropapillary carcinoma of the breast. Mod. Pathol. 6, 660–662 (1993).
3. Tavassoli, F. A. & Devilee, P. Tumours of the Breast and Female Genital Organs. WHO Classification of Tumours (IARC Press, 2003).
4. Amin, M. B. et al. Micropapillary variant of transitional cell carcinoma of the urinary bladder. Histologic pattern resembling ovarian papillary serous carcinoma. Am. J. Surg. Pathol. 18, 1224–1232 (1994).
5. Sakamoto, K. et al. Primary invasive micropapillary carcinoma of the colon. Histopathology 47, 479–484 (2005).
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