Puf6 primes 60S pre-ribosome nuclear export at low temperature

Author:

Gerhardy Stefan,Oborská-Oplová MichaelaORCID,Gillet LudovicORCID,Börner RichardORCID,van Nues RobORCID,Leitner AlexanderORCID,Michel Erich,Petkowski Janusz J.ORCID,Granneman Sander,Sigel Roland K. O.ORCID,Aebersold RuediORCID,Panse Vikram GovindORCID

Abstract

AbstractProductive ribosomal RNA (rRNA) compaction during ribosome assembly necessitates establishing correct tertiary contacts between distant secondary structure elements. Here, we quantify the response of the yeast proteome to low temperature (LT), a condition where aberrant mis-paired RNA folding intermediates accumulate. We show that, at LT, yeast cells globally boost production of their ribosome assembly machinery. We find that the LT-induced assembly factor, Puf6, binds to the nascent catalytic RNA-rich subunit interface within the 60S pre-ribosome, at a site that eventually loads the nuclear export apparatus. Ensemble Förster resonance energy transfer studies show that Puf6 mimics the role of Mg2+ to usher a unique long-range tertiary contact to compact rRNA. At LT, puf6 mutants accumulate 60S pre-ribosomes in the nucleus, thus unveiling Puf6-mediated rRNA compaction as a critical temperature-regulated rescue mechanism that counters rRNA misfolding to prime export competence.

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry

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