Abstract
AbstractWith the increasing global demand for meat, cultured meat technologies are emerging, offering more sustainable solutions that aim to evade a future shortage of meat. Here, we demonstrate a cultured meat platform composed of edible microcarriers and an oleogel-based fat substitute. Scalable expansion of bovine mesenchymal stem cells on edible chitosan-collagen microcarriers is optimized to generate cellularized microtissues. In parallel, an oleogel system incorporated with plant protein is developed as a fat substitute, which is comparable to beef fat in appearance and texture. Combining the cellularized microtissues with the developed fat substitute, two types of cultured meat prototypes are introduced: layered cultured meat and burger-like cultured meat. While the layered prototype benefits enhanced stiffness, the burger-like prototype has a marbling meat-like appearance and a softer texture. Overall, this platform and the established technological basis may contribute to the development of different cultured meat products and promote their commercial production.
Funder
Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology
The Good Food Institute
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
Reference33 articles.
1. Bhat, Z. F., Kumar, S. & Bhat, H. F. In vitro meat: a future animal-free harvest. Crit. Rev. Food Sci. Nutr. 57, 782–789 (2017).
2. Hong, T. K., Shin, D.-M., Choi, J., Do, J. T. & Han, S. G. Current issues and technical advances in cultured meat production: a review. Food Sci. Anim. Resour. 41, 355–372 (2021).
3. Van Wezel, A. L. Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nat. 1967 2165110 216, 64–65 (1967).
4. Tsai, A.-C., Jeske, R., Chen, X., Yuan, X. & Li, Y. Influence of microenvironment on mesenchymal stem cell therapeutic potency: from planar culture to microcarriers. Front. Bioeng. Biotechnol. 8, 640 (2020).
5. Bodiou, V., Moutsatsou, P. & Post, M. J. Microcarriers for upscaling cultured meat production. Front. Nutr. 7, 1–16 (2020).
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献