A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
Link
http://www.nature.com/articles/s41467-019-09005-3.pdf
Reference27 articles.
1. Stovicek, V., Holkenbrink, C. & Borodina, I. CRISPR/Cas system for yeast genome engineering: advances and applications. FEMS Yeast Res. 17, fox030 (2017).
2. Khan, S. et al. CRISPR/Cas9: the Jedi against the dark empire of diseases. J. Biomed. Sci. 25, 29 (2018).
3. Bortesi, L. & Fischer, R. The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnol. Adv. 33, 41–52 (2015).
4. Zhao, D. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb. Cell Fact. 15, 205 (2016).
5. Jakočiūnas, T. et al. Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae. Metab. Eng. 28, 213–222 (2015).
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